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以番茄灰霉病菌(Botrytis cinerea)为供试菌,采用平板对峙方法,研究了不同植物根围土壤中的细菌对番茄灰霉病(Botrytis cinerea)的拮抗效果。结果表明:对分离纯化的75株细菌进行拮抗测定,其中30株对番茄灰霉病有不同程度抑制作用,分离频率为40.00%。其中3株拮抗细菌YM8、FQ10、FQ11对番茄灰霉病病原菌拮抗效果较好,抑菌圈直径在1.5~3.0cm。通过生理生化试验、形态特征观察对其进行了种属初步鉴定,鉴定结果为菌株YM8为地衣芽孢杆菌(Bacillus licheniformis),FQ10为短芽孢杆菌(Bacillus brevis),FQ11为蜡状芽孢杆菌(Bacillus cereus)。
Botrytis cinerea was used as the test bacterium to study the antagonistic effect of bacteria in the rhizosphere soil of different plants on Botrytis cinerea by the slab confrontation method. The results showed that 75 strains isolated and purified were tested for their antagonism. Among them, 30 strains had different degrees of inhibition against Botrytis cinerea, with a frequency of 40.00%. Three antagonistic bacteria YM8, FQ10 and FQ11 had better antagonistic effect on Botrytis cinerea, and the diameter of bacteriostatic circle was between 1.5 and 3.0 cm. The results showed that the strain YM8 was Bacillus licheniformis, FQ10 was Bacillus brevis, FQ11 was Bacillus cereus (Bacillus cereus) ).