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自杂交水稻研究50年来,红莲型杂交水稻采取基础研究、应用研究、产业化相结合的模式为杂交水稻事业的发展做出重要贡献.红莲型细胞质雄性不育基因orf H79位于线粒体基因atp6下游,编码79个氨基酸,具细胞毒性,与电子传递链复合体Ⅲ的蛋白亚基互作,机理研究结果表明这种互作可能引起ROS的上升而导致花粉败育.对两个育性恢复基因Rf5,Rf6的研究结果表明,这两个PPR基因均不能与不育基因转录本直接互作,其中RF5与GRP162及RFC3等蛋白互作,以蛋白复合体的形式加工不育基因转录本,而RF6与HXK6互作,形成新的蛋白复合体,二者育性恢复通路各自独立.以不育基因orf H79为分子标记,创制出一批新的红莲型细胞质不育系;以恢复基因Rf5,Rf6为分子标记,筛选出一批双恢复基因强恢复系,选育出高产优质多抗广适红莲型杂交水稻新组合红莲优6,粤优938和珞优8号等.红莲型杂交稻在中国长江流域、华南稻区大面积推广种植,同时在越南、印度尼西亚、马来西亚、孟加拉和菲律宾等东南亚国家引种推广并表现优异,展现出广阔的应用前景.
Since hybrid rice research for 50 years, Honglian hybrid rice has made a significant contribution to the development of hybrid rice through basic research, applied research and industrialization mode. The orf H79 gene of Honglian type cytoplasmic male sterility is located at mitochondrial gene atp6 Downstream, it encodes a protein of 79 amino acids, which is cytotoxic and interacts with the protein subunits of the electron transport chain complex III. The results of the mechanism studies show that this interaction may cause the rise of ROS and lead to pollen abortion. The results of Rf5 and Rf6 showed that neither of the two PPR genes interacted directly with the sterile gene transcripts. RF5 interacted with proteins such as GRP162 and RFC3 to process sterile transcripts in the form of protein complexes, RF6 interacts with HXK6 to form a new protein complex, and the two fertility restoration pathways are independent.With the sterile gene orf H79 as a molecular marker, a new batch of HLK cytoplasmic male sterile lines was created, Rf5 and Rf6 were selected as molecular markers, and a number of strong restorer lines with double restorer genes were screened out, and the new combinations of high yield and high quality polyclonal to Hongliangyou hybrid rice, such as Hongliangyou 6, Yueyou 938 and Luoyou 8, Lian-type hybrid rice in Yangtze River basin countries, large-scale planting rice in South China, while promoting the introduction and outstanding performance in the Southeast Asian country of Vietnam, Indonesia, Malaysia, Bangladesh and the Philippines, show a broad application prospects.