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目的:通过与VC的对比实验,研究虾青素的抗氧化能力。方法:分别采用DPPH自由基测定法、水杨酸法以及邻苯三酚自氧化法,研究虾青素和VC在清除DPPH自由基(DPPH·)、羟基自由基(·OH)和超氧阴离子(O2-·)的能力。结果:虾青素能有效地抑制羟基自由基、超氧阴离子自由基,且清除率大于VC,清除DPPH·自由基方面虽然略低于VC,但同样表现出较高的清除率,当浓度为90μg/mL时,清除率为95.0%。结论:虾青素具有明显的抗氧化活性。
OBJECTIVE: To study the antioxidant capacity of astaxanthin by comparing with VC. Methods: DPPH radical scavenging assay, salicylic acid method and pyrogallol autoxidation method were used to study the effects of astaxanthin and VC on scavenging DPPH ·, · OH and superoxide anion (O2- ·) ability. Results: Astaxanthin can effectively inhibit hydroxyl radicals and superoxide anion radicals, and its clearance rate is higher than that of VC. Although scavenging DPPH · free radicals slightly lower than VC, astaxanthin also showed a higher clearance rate. When the concentration was At 90 μg / mL clearance was 95.0%. Conclusion: Astaxanthin has obvious antioxidant activity.