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乳腺针吸检查具有操作方便、伤口小、痛苦轻等优点,因此易于被女性病人接受.乳腺局部雌激素的合成主要依靠芳香化酶和硫酸酯酶这两条途径,在乳腺癌变过程中起重要作用.课题组前期研究证实,巢式定量PCR在检测乳腺组织芳香化酶时具有较高的灵敏度和特异性.目前对于乳腺细针穿刺得到的微量组织中芳香化酶和硫酸酯酶表达水平的了解很少,本研究对74例良恶性乳腺针吸组织进行芳香化酶和硫酸酯酶mRNA表达水平的检测,同时对比了巢式和非巢式PCR方法应用到针吸组织时的检测功效.结果发现,巢式和非巢式PCR方法在检测乳腺针吸组织芳香化酶mRNA表达水平上没有差异.mRNA表达水平和病人临床资料相关性分析后发现,年龄和芳香化酶表达水平是乳腺癌发生的两个独立危险因素(P=0.04和P=0.00),月经状态和硫酸酯酶mRNA表达水平与乳腺癌的发生不相关.因此本研究提示,巢式和非巢式PCR对于针吸组织的研究均有效,年龄和芳香化酶表达水平,而非月经状态和硫酸酯酶表达水平,是乳腺癌发生的危险因素.
Breast needle aspiration examination has the advantages of convenient operation, small wound, light pain, etc., so it is easy to be accepted by female patients. The synthesis of estrogen mainly depends on both aromatase and sulfatase, and plays an important role in breast carcinogenesis The preliminary study of the research group confirmed that nested quantitative PCR has high sensitivity and specificity in the detection of aromatase in breast tissue.Nowadays, the expression of aromatase and sulfatase in microstructure obtained by fine needle aspiration Little is known about the detection of aromatase and sulfatase mRNA expression in 74 cases of benign and malignant breast acupuncture tissues. In addition, the detection efficiency of nested and non-nested PCR methods applied to needle aspiration tissue was compared. The results showed that there was no difference in detecting the expression of aromatase mRNA between breast and needle aspiration by nested and non-nested PCR.Analysis of mRNA expression and clinical data showed that age and aromatase expression level were significantly higher than those of breast cancer There were two independent risk factors (P = 0.04 and P = 0.00), the menstrual status and the expression level of sulfatase mRNA were not correlated with the occurrence of breast cancer.Therefore, Both nested and non-nested PCR were effective in studying needle aspiration, age, and aromatase expression, but not in menstrual status and sulfatase expression, as risk factors for breast cancer.