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氚水诱发F_1代小鼠肿瘤实验研究,分三个剂量组,即22.2×10~7Bq×4,11.1×10~7Bq×4,3.7×10~7Bq×4和一个对照组。结果显示,最高剂量组动物,雌性小鼠肿瘤发生率为57.1％,雄性小鼠肿瘤发生率为39.3％,肿瘤发生潜伏期分别为195.3±77.4天和180.3±57.1天。肿瘤主要类型是淋巴细胞白血病和胸腺瘤。原代金黄地鼠胚胎细胞,在体外培养条件下,经放射性比活度1mci/ml和2.5mci/mlHTO作用后,24～26天潜伏期细胞发生了转化,在无刺激因子条件下,半琼脂培养能形成细胞集落。L_(855)白血病细胞系,在不同放射性比活度0.66×10~7～23.125×10~7Bq/mlHTO作用后,其微核细胞率和微核率与放射性比活度呈线性关系,回归方程分别是Y=0.8183+0.1416X,r=0.98和Y=0.0508+0.1548X,r=0.96。小鼠一次腹腔注入放射性比活度为518MBq的氚水后20小时,小肠上皮坏死细胞达到峰值(5.48个/隐窝),然后开始减少,当注入37～555MBq不同放射性比活度的氚水后6小时,小肠隐窝上皮坏死细胞与放射性比活度呈线性关系。Y=0.0263+0.1612X,r=0.99。
Drowning water induces F 1 generation mice tumor experimental study, divided into three dose groups, namely 22.2 × 10 ~ 7Bq × 4, 11.1 × 10 ~ 7Bq × 4, 3.7 × 10 ~ 7Bq × 4 and a control group. The results showed that the highest tumor dose was 57.1% in females and 39.3% in males, and the tumor incubation latency was 195.3±77.4 days and 180.3±57.1 days, respectively. The main types of cancer are lymphocytic leukemia and thymoma. The primary golden hamster embryo cells, under in vitro culture conditions, undergo a specific radioactivity of 1 mci/ml and 2.5 mci/ml HTO, and the cells in the incubation period of 24 to 26 days were transformed. Under the condition of no stimulation, semi-agar culture Can form cell colonies. In the L_(855) leukemia cell line, the micronuclear cell rate and micronucleus rate were linearly related to the specific radioactivity after different radiobiological activity of 0.66×10~7~23.125×10~7Bq/ml HTO. It is Y=0.8183+0.1416X, r=0.98 and Y=0.0508+0.1548X, r=0.96, respectively. After 20 hours of intraperitoneal injection of deuterium with a specific radioactivity of 518 MBq, the necrotic cells in the small intestine epithelium reached a peak (5.48 cells/crypt), and then they began to decrease after injecting 37-555 MBq of different radioactivity into the water. At 6 hours, there was a linear relationship between necrotic cells in the intestinal crypt epithelium and specific radioactivity. Y=0.0263+0.1612X, r=0.99.