目的:探讨Sam68蛋白对白血病细胞Jurkat凋亡的影响。方法:针对Sam68靶点构建pL KO-Tet-On条件性真核表达干扰载体,制备慢病毒并感染Jurkat细胞,诱导干扰载体表达,筛选稳定表达shRNA细胞;采用实时荧光定量和免疫印迹技术检测干扰效率;应用流式细胞术检测细胞凋亡,采用免疫印迹技术检测凋亡相关蛋白表达水平变化情况。结果:Sam68基因表达下 Objective: To investigate the effect of Sam68 protein on Jurkat apoptosis in leukemia cells. Methods: The eukaryotic expression vector pLKO-Tet-On was constructed based on the target of Sam68. The lentivirus was infected and infected with Jurkat cells. The expression of the interfering vector was induced and the shRNA cells were stably expressed. The real-time fluorescent quantitative and immunoblotting methods were used to detect the interference The apoptosis rate was detected by flow cytometry. The expression of apoptosis-related proteins was detected by Western blotting. Results: Sam68 gene expression