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目的 通过研究未化疗的原发性胰腺癌mdrl基因mRNA及其蛋白产物p-gP的表达与肿瘤生物学特性的关系,以便指导胰腺癌的临床治疗。方法 应用免疫组织化学、原位PCR技术对150例胰腺石蜡包埋标本(包括97例原发胰腺癌,32例胰腺炎及21例正常胰腺组织)组织中的mdrl mRNA和P —gp进行检测。结果 mdrl mRNA和p-gP染色主要分布于正常胰腺小导管及肿瘤的导管上皮细胞的细胞膜和/或细胞浆内。胰腺癌和胰腺炎及正常胰腺组织中mdrl mRNA和P-gp表达阳性率之间存在着显著性差异(P<0.05),mdrl mRNA和P—gP的阳性表达率与肿瘤的分化程度、浸润性和TNM分期等生物学特性有关(P<0.05),而与病人的年龄、性别、肿瘤的大小及部位等临床病理资料无关(P>0.05)。结论 研究结果表明胰腺癌mdrl基因的表达与其“天然”耐药有关,检测胰腺癌mdrl基因表达对预测胰腺癌的化疗敏感性具有重要指导意义。同时它可能作为判断肿瘤的恶性程度及预后的一个有效生物学指标。
Objective To study the relationship between the expression of mdrl gene mRNA and its protein product p-gp in non-chemotherapy primary pancreatic cancer and its biological characteristics so as to guide the clinical treatment of pancreatic cancer. Methods Immunohistochemistry and in situ PCR were used to detect mdrl mRNA and P-gp in 150 cases of pancreatic paraffin-embedded specimens (including 97 cases of primary pancreatic cancer, 32 cases of pancreatitis and 21 cases of normal pancreatic tissue). Results The mdrl mRNA and p-gp staining were mainly located in the cell membrane and / or cytoplasm of ductal epithelial cells in normal pancreatic duct and tumor. The positive rates of mdrl mRNA and P-gp in pancreatic cancer, pancreatitis and normal pancreatic tissues were significantly different (P <0.05). The positive rates of mdrl mRNA and P-gp were significantly correlated with the degree of tumor differentiation, invasiveness And TNM stage (P <0.05), but not with clinicopathological data such as age, sex, tumor size and site (P> 0.05). Conclusion The results show that mdrl gene expression in pancreatic cancer is related to its “natural” drug resistance. Detecting mdrl gene expression in pancreatic cancer is important for predicting the chemosensitivity of pancreatic cancer. At the same time it may be used as a valid biological indicator to judge the malignant degree and prognosis of the tumor.