论文部分内容阅读
目的 探讨血红素氧合酶 1(hemeoxygennase 1,HO 1)在妊娠期高血压疾病患者中的表达与作用机制。方法 2 0 0 2年 6月至 2 0 0 3年 6月间 ,甘肃省妇幼保健院采用分光光度法测定 34例妊娠期高血压疾病患者和 2 0例正常晚孕妇女血清的HO 1活性及血浆中的碳氧血红蛋白 (HbCO)的百分含量 ,同时采取胎盘做HO 1的免疫组化。结果 妊娠期高血压组HO 1活性及HbCO与对照组比较差异无显著性 (P >0 0 5 ) ;而子疒间 前期及子疒间 组与对照组比较则差异有显著性意义 (P <0 0 5 )。血浆HbCO的百分含量的变化与血清HO 1的活性变化呈正相关。胎盘中子疒间 前期及子疒间组患者的蛋白质表达升高 (P <0 0 5 ) ,而妊娠期高血压组与对照组差异无显著性 (P >0 0 5 )。结论 妊娠期高血压疾病患者血清HO 1活性及在胎盘中表达的变化与妊娠高血压疾病发病有关 ,对其的检测可能作为血管及胎盘细胞损伤的指标之一。
Objective To investigate the expression and mechanism of hemeoxygennase 1 (HO 1) in patients with hypertensive disorder complicating pregnancy. Methods From June 2002 to June 2003, Gansu Provincial Maternal and Child Health Hospital measured the HO 1 activity of 34 cases of hypertensive disorder complicating pregnancy and 20 normal pregnant women by spectrophotometry. Plasma carboxyhemoglobin (HbCO) percentage, while taking the placenta HO 1 immunohistochemistry. Results There was no significant difference in HO 1 activity and HbCO between the prehypertensive group and the control group (P> 0.05), but there was significant difference between the prehypnotic group and the control group (P < 0 0 5). Changes in the percentage of plasma HbCO and serum HO 1 activity was positively correlated. The protein expression of placental neutrons in patients with preeclampsia and subependymal was elevated (P <0.05), but there was no significant difference between gestational hypertension and controls (P> 0.05). Conclusions The changes of serum HO 1 activity and placenta expression in patients with hypertensive disorder complicating pregnancy are related to the pathogenesis of pregnancy-induced hypertension. The detection of HO 1 may be one of the indicators of vascular and placental cell damage.