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近几年转基因大豆的品种不断增加,以大豆为原料的食品也进入到食品供应链中,对于转基因大豆及其衍生食品的定量检测可以为转基因大豆的商业化和安全性提供重要保证。以PCR为基础的转基因大豆定量检测可以提供产品的转基因含量,具体表示方式为外源基因拷贝数与大豆基因拷贝数的比值。该比值仅当所有大豆品系(包括转基因品系)的基因拷贝数一致时才有效。本研究考察了15种大豆(Glycine max L.)品系不同组织(胚、子叶和种皮)的DNA含量以及DNA浓度和核酸DNA含量对转基因定量检测的影响。结果表明,DNA浓度不能简单使用转基因样品与总样品
In recent years, the varieties of genetically modified soybeans continue to increase, and soy-based foods have also entered the food supply chain. The quantitative detection of genetically modified soybeans and their derived foods can provide an important guarantee for the commercialization and safety of genetically modified soybeans. Quantitative PCR-based detection of genetically modified soybeans can provide transgenic content of the product as a ratio of the copy number of the foreign gene to the copy number of the soybean gene. This ratio is valid only if the gene copy number of all soybean lines (including transgenic lines) is consistent. In this study, we investigated the DNA contents of different tissues (embryo, cotyledon and seed coat) of 15 soybean (Glycine max L.) strains as well as the effect of DNA concentration and DNA content on the quantitative detection of transgene. The results show that the DNA concentration can not simply use transgenic samples and total samples