论文部分内容阅读
目的探讨人卵裂期胚胎和囊胚玻璃化冷冻解冻后复苏效果及妊娠结局。方法回顾性分析434个卵裂期胚胎(卵裂组)和102个囊胚解冻周期(囊胚组)的临床资料,比较采用玻璃化冷冻解冻后的复苏率及妊娠结局。结果囊胚组患者的临床妊娠率和胚胎种植率分别为63.73%(65/102)和45.79%(87/190),卵裂组分别为42.82%(185/432)和24.17%(241/997),两组比较,差异均有统计学意义(P均<0.05);囊胚组患者的复苏率、流产率和异位妊娠率分别为98.45%(190/193)、12.31%(8/65)和0(0/65),卵裂组分别为97.55%(997/1 022)、13.51%(25/185)和2.16%(4/185),两组比较,差异均无统计学意义(P均>0.05)。卵裂组中,卵裂球完全存活患者的临床妊娠率和胚胎种植率分别为44.62%(170/381)和25.57%(224/876),非完全存活者分别为29.41%(15/51)和14.05%(17/121),两组比较,差异均有统计学意义(P均<0.05)。结论囊胚解冻移植的妊娠结局优于卵裂期胚胎,卵裂期胚胎解冻后胚胎的完整性影响胚胎种植。
Objective To investigate the effect of resuscitation and pregnancy outcome after vitrification and thawing of human cleavage stage embryos and blastocysts. Methods The clinical data of 434 cleavage embryos (cleavage group) and 102 blastocysts (blastocyst group) were retrospectively analyzed. The recovery rate and pregnancy outcome after vitrification were compared. Results The clinical pregnancy rate and embryo implantation rate of the blastocyst group were 63.73% (65/102) and 45.79% (87/190), respectively. The rate of cleavage was 42.82% (185/432) and 24.17% (241/997) ), The difference between the two groups was statistically significant (P <0.05). The recovery rate, abortion rate and ectopic pregnancy rate in the blastocyst group were 98.45% (190/193), 12.31% (8/65) ) And 0 (0/65), cleavage group 97.55% (997/1 022), 13.51% (25/185) and 2.16% (4/185) respectively. There was no significant difference between the two groups P> 0.05). In the cleavage group, the clinical pregnancy rate and embryo implantation rate in patients with complete survival of blastomeres were 44.62% (170/381) and 25.57% (224/876), respectively, and those with incomplete survival were 29.41% (15/51) And 14.05% (17/121) respectively. There was significant difference between the two groups (all P <0.05). Conclusion The outcome of pregnancy after blastocyst thawing is better than that of cleavage stage embryo. The integrity of embryo after thawing of cleavage stage embryos affects embryo implantation.