目的 :建立反相高效液相色谱法测定滇桂艾纳香中原儿茶酸的方法。方法 :滇桂艾纳香水提液醋酸乙酯 乙醇 (4∶1)提取 ,薄层色谱分离 ,反相高效液相色谱法测定 ,μbondapakC18色谱柱 ,流动相甲醇 水 冰醋酸 (19∶80∶1) ,检测波长 2 6 0nm。结果 :进样量 3.31～ 41.8μg·ml-1,线性关系良好 (r =0 .9999) ,加样回收率为 98.0 5 % ,RSD=1.94% (n =6 )。结论 :该法为开发利用滇桂艾纳香提供依据 Objective : To establish a reversed-phase high performance liquid chromatography method for the determination of protocatechuic acid in A. fruticosa. Method: Acer ginnala Perspex extract ethyl acetate ethanol (4:1) extraction, thin layer chromatography, reversed-phase high performance liquid chromatography, μbondapak C18 column, mobile phase methanol water glacial acetic acid (19:80:1) ), detection wavelength 260nm. Results : The injection volume was 3.31～41.8μg·ml-1, the linear relationship was good (r=0.9999), the recovery rate was 98.05%, RSD=1.94% (n=6). Conclusion : This law provides the basis for the development and utilization of A. grandis