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目的 对实验性糖尿病大鼠 (STZ- Wistar大鼠 )心肌组织血管紧张素 (Ag- )含量及 Ag- 受体亚型 1(AT1受体 )基因 m RNA表达和卡托普利 (血管紧张素转换酶抑制剂 ,ACEI)应用对心脏病变可能的保护作用进行了观察。方法 应用分子生物学和放射免疫的方法。结果 未经胰岛素治疗的糖尿病大鼠 (DM组 )在 4个月病程时 ,心重 /体重 (HW/ BW)比值增加 ,心脏组织中 Ag- 含量 DM组高于正常对照组 (N组 ) ,有显著性差异。 DM组 AT1受体基因m RNA表达亦明显增强 ,显示为升调节。在经卡托普利治疗的 DM- C组 ,虽同样因糖尿病影响体重低于 N组 ,HW/ BW比值及心脏组织 Ag- 含量和 AT1基因 m RNA表达则保持与 N组相近 ,且低于 DM组。结论 ACEI的治疗对糖尿病大鼠心脏病变有保护性效应。
Objective To investigate the expression of angiotensin (Ag) and mRNA of Ag-receptor subtype 1 (AT1 receptor) gene in experimental diabetic rats (STZ-Wistar rats) and the effects of captopril (angiotensin Conversion enzyme inhibitors, ACEI) application of the possible protective effect of heart disease were observed. Methods Using molecular biology and radioimmunoassay methods. Results Compared with the normal control group (N group), the diabetic rats (DM group) without insulin treatment had an increased HW / BW ratio at 4-month duration and a higher Ag- Significant difference. DM group AT1 receptor gene m RNA expression was also significantly enhanced, showed up regulation. In the DM-C group treated with captopril, the HW / BW ratio, the Ag-content in cardiac tissue and the mRNA expression of AT1 gene remained similar to those in group N, although they were also lower than those in group N due to diabetes mellitus DM group. Conclusion The treatment of ACEI has a protective effect on heart disease in diabetic rats.