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[目的]探讨太白楤木对成纤维细胞增殖及形态学的影响及抗肝纤维化机制。[方法]采用体外培养的NIH3T3成纤维细胞作为肝星状细胞(HSC)的替代模型,常规培养,秋水仙碱、齐墩果酸及太白楤木中药血清作用于细胞,在光镜及电镜下观察药物血清对NIH3T3细胞形态学的影响;用放射免疫法测定培养上清透明质酸(HA)的生成。[结果]太白楤木、齐墩果酸、秋水仙碱血清组细胞与对照组细胞在形态、数目上差别显著;3者均可抑制细胞增殖(P<0.01);显著抑制HA的合成,降低NIH3T3细胞培养上清中HA水平,与对照组比较差异有统计学意义(P<0.01)。[结论]太白楤木中药血清可显著抑制NIH3T3细胞增殖和细胞外HA的合成,在体外具有一定的抗肝纤维化作用。
[Objective] To study the effects of Alnus cremastogyne on the proliferation and morphology of fibroblasts and the mechanism of anti-fibrosis. [Methods] NIH3T3 fibroblasts cultured in vitro were used as an alternative model of hepatic stellate cells (HSC). In routine culture, serum of colchicine, oleanolic acid, and Taibai Hemu was applied to cells under light and electron microscopes. The effect of drug serum on the morphology of NIH3T3 cells was observed; the production of hyaluronic acid (HA) in culture supernatant was measured by radioimmunoassay. [Results] The morphological and the number of cells in the serogroups of T. chinensis, oleanolic acid, and colchicine groups were significantly different from each other; all of them could inhibit cell proliferation (P<0.01), and significantly inhibited the synthesis of HA and decreased The HA level in the culture supernatant of NIH3T3 cells was significantly different from that of the control group (P<0.01). [Conclusion] The serum of Taibai Chinese wolfberry wood can significantly inhibit the proliferation of NIH3T3 cells and the synthesis of extracellular HA, and has a certain anti-fibrotic effect in vitro.