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试验结果表明:影响茶尺蠖核型多角体病毒产量最主要的因子是饲毒浓度,其次是饲毒时宿主幼虫的虫龄,再是饲毒浓度与虫龄的组合,在4龄初期采用2.2×10~6PIB/毫升的病毒浓度饲毒48小时得到了最高产量。 在15℃、20℃、25℃、30℃,35℃下饲毒、饲养的结果表明:随着温度上升,病毒产量提高,25℃时产量最高,30℃,35℃依次下降,30℃下死亡率低,化蛹多,说明高温能抑制病毒的增殖,35℃下死亡率虽然很高,但病毒产量最低,在23℃下饲毒48小时后分别在前述五种温度下饲养,病毒产量的变化趋势相同,唯产量均比前者为高。 在6:18、9:15、12:12、15:9、18:6(光:暗,小时)光周期,下饲毒、饲养,病毒产量以9:15为最高,以后随着光照时间的延长,产量依次下降。 不同虫龄、虫态病死虫的多角体含量比较结果,以5龄中后期死亡的幼虫含量最高,依次是预蛹、蛹、3龄末4龄初的病死虫。
The results showed that the main factors influencing the yield of polyhedrosis virus were the feeding concentration, followed by the larvae of host larvae and the combination of feeding concentration and larvae age. × 10 ~ 6PIB / ml virus concentration of 48 hours to get the highest yield. The results showed that the yield of virus increased with the increase of temperature, the highest yield was at 25 ℃, the lowest was at 30 ℃ and the latter at 35 ℃, while the lowest at 30 ℃ Low mortality, pupation and more, indicating that high temperature can inhibit the proliferation of the virus, although the mortality at 35 ℃ is very high, but the lowest virus yield, at 48 ℃ for 48 hours after feeding at the above five temperatures, the virus yield The same trend of change, only the production is higher than the former. In 6: 18,9: 15,12: 12,15: 9,18: 6 (light: dark, hour) photoperiod, feeding, feeding, the virus yield to 9:15 for the highest, then with the light time Of the extension, the output decreased in turn. The results showed that the larvae dead at the fifth instar larvae had the highest levels of larvae, followed by pre-pupae, pupae, and diseased larvae at the end of the third instar and the fourth instar larvae.