论文部分内容阅读
目的探讨六味地黄方体内外对草酸钙结石形成的影响。方法采用种晶技术检测六味地黄方不同质量浓度(20、10、5、1 mg/mL)在体外对(Coo)2Ca.H2O晶体生长的抑制指数。60只SD大鼠随机分为6组,对照组,模型组,阳性对照组,六味地黄方高、中、低剂量(0.8、0.4、0.2 g/kg)组。饲养4周后,检测各组大鼠血生化指标,24 h尿草酸量,Ca2+、Mg2+分泌量和肾组织Ca2+、Mg2+水平。镜下观察各组大鼠肾组织切片中草酸钙结晶分布及组织病理改变。结果体外实验中,20、10、5 mg/mL六味地黄方对结晶生长抑制指数分别为98.50%、89.20%、43.94%,明显高于对照组(P<0.05)。体内实验,六味地黄方高剂量组大鼠血清尿素氮(BUN)、肌酐(SCr)水平,肾Ca2+水平,24 h尿Ca2+分泌量及肾组织草酸钙结晶沉积均低于模型组与阳性对照组(P<0.05)。结论六味地黄方具有抑制草酸钙结石形成的作用,并且其抑制效果优于枸橼酸钾。
Objective To investigate the effect of Liuwei Dihuang decoction on the formation of calcium oxalate stones. Methods The growth inhibition index of (Coo) 2Ca.H2O crystals in vitro treated with different concentrations of 20, 10, 5 and 1 mg / mL of Liuweidihuang was detected by seeding technique. Sixty Sprague-Dawley rats were randomly divided into 6 groups: control group, model group, positive control group, and Liuweidihuang high, medium and low dose groups (0.8, 0.4 and 0.2 g / kg). After feeding for 4 weeks, the blood biochemistry indexes of rats in each group were tested, the quantity of urinary oxalate, the secretion of Ca2 + and Mg2 + and the levels of Ca2 + and Mg2 + in renal tissue were measured. Microscopically observe the distribution and histopathological changes of calcium oxalate crystals in the kidney sections of rats in each group. Results In vitro, the inhibitory indexes of crystal growth of Liuwei Dihuang Formula 20,10,5 mg / mL were 98.50%, 89.20% and 43.94%, respectively, which were significantly higher than that of the control group (P <0.05). In vivo, the levels of serum urea nitrogen (BUN) and creatinine (SCr), renal Ca2 +, 24 h urinary Ca2 + secretion and kidney calcium oxalate deposition were significantly lower in the Liuwei Dihuang Fang high-dose group than in the model group and the positive control group (P <0.05). Conclusion Liuweidihuang prescription can inhibit the formation of calcium oxalate stones, and its inhibitory effect is better than potassium citrate.