论文部分内容阅读
小白鼠是遗传学和药物毒理研究常用的动物之一。我们用小鼠骨髓直接制备染色体,不需要复杂和严格的培养条件,此方法简单、快速、分散程度良好,染色体清晰。操作方法介绍如下: 一、主要试剂: 1.2％柠檬酸钠。 2.秋水仙素:配成0.05％秋水仙素溶液,冰箱保存。 3.低渗液:0.3％氯化钾溶液。 4.固定液:1:3的冰醋酸和甲醇,临用现配。 5:磷酸盐缓冲液。 6.姬姆萨染液。二、方法: 1.用无菌注射器,在小鼠腹腔注入0.05％秋水仙素,按体重3～4毫克/公斤。 2.注入秋水仙素3～4小时后,拉颈椎致死。
Mice are one of the most commonly used animals in genetics and drug toxicology research. We use mouse bone marrow to directly prepare chromosomes without the need for complicated and rigorous culture conditions. This method is simple, rapid, well-dispersed and clear in chromosomes. Operating methods are described below: First, the main reagent: 1.2% sodium citrate. Colchicine: dubbed 0.05% colchicine solution, the refrigerator to save. 3. Low permeability liquid: 0.3% potassium chloride solution. 4 fixed solution: 1: 3 glacial acetic acid and methanol, Pro use now. 5: Phosphate buffer. 6. Giemsa dye solution. Second, methods: 1. With a sterile syringe, intraperitoneal injection of 0.05% colchicine, according to the weight of 3 to 4 mg / kg. 2 injection of colchicine 3 to 4 hours after pulling the cervical spine death.