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目的:优选盾叶薯蓣胚性愈伤诱导的最佳培养基组成。方法:采用茎尖分生组织进行离体培养获取盾叶薯蓣试管苗。切取试管苗不同部位诱导胚状体和愈伤组织,筛选最佳外植体;以生长率和薯蓣皂苷元含量作为指标,采用正交实验筛选胚性愈伤诱导培养基的最佳激素配比。结果:诱导愈伤组织和胚状体的最佳外植体为试管苗叶片,诱导率分别为92.5%和42.5%;胚性愈伤诱导最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L。结论:本研究结果可实现盾叶薯蓣胚性愈伤的有效诱导,为后续人工种子研究提供基础依据。
Objective: To optimize the embryo callus induction of Dioscorea zingiberensis medium composition. Methods: In vitro culture of shoot apical meristem was used to obtain Dioscorea bulbifera plantlets. The embryoid body and callus were induced from different parts of the vitro plantlets and the best explants were screened. The growth rate and diosgenin content were used as indexes to screen the best hormonal proportion of embryogenic callus induction medium . Results: The best explants for inducing callus and embryoid body were as follows. The induction rate of the explants was 92.5% and 42.5%, respectively. The best medium for embryogenic callus induction was MS + 6-BA 2.0 mg / L + NAA 0.5 mg / L + 2,4-D 1.0 mg / L. Conclusion: The results of this study can effectively induce the embryogenic callus of Dioscorea zingiberensis, providing the basis for further study on artificial seeds.