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目的:探讨白花蛇舌草水提取物、醇提取物对LPS所致脓毒症模型小鼠炎症的作用及其可能的免疫学机制.方法:将小鼠随机分为空白对照组、模型组、地塞米松干预组和白花蛇舌草水提组、醇提组.地塞米松干预组、水提组、醇提组连续灌胃7天, 其余各组给予等体积生理盐水;末次给药30min后, 除空白组外, 其余各组小鼠腹腔注射LPS (10mg/kg) 造模, 空白组给予等体积生理盐水, 1次/天持续至实验结束.分别于造模后6h、12h、24h、48h进行临床症候观察, 酶联免疫吸附法 (ELISA) 检测小鼠血清炎症因子白介素-6 (IL-6) 、白介素-1β (IL-1β) 和肿瘤坏死因子-α (TNF-α) 水平, 光镜下观察小鼠肝脏组织病理学变化.结果:与同时间节点模型组比较, 白花蛇舌草水提组和白花蛇舌草醇提组小鼠血清IL-6、IL-1β、TNF-α水平明显降低 (P<0.05).组内比较, 白花蛇舌草提取物组12h节点对上述指标的改善均明显优于6h节点, 并且与白花蛇舌草醇提取物组相比, 白花蛇舌草水提取物组各时间节点作用效果更好, 但无显著性差异.病理学结果显示, 白花蛇舌草提取物组小鼠肝脏组织损伤程度明显轻于模型组, 并且水提组损伤减轻更明显.结论:白花蛇舌草提取物能够有效改善LPS脓毒症小鼠的肝损伤, 抑的制平I衡L-而6发、IL挥-1作β、用TN.F-α水平, 其作用机制可能是通过抑制此相关炎症因子的表达, 调节固有免疫系统的平衡而发挥作用.“,”Objective:To investigate the inhibitory effect of aqueous extracts and alcohol extracts of Hedyotis Diffusa Herba (EEHDH) on the inflammation of sepsis mice and its immunological mechanism.Methods:The mice were randomly divided into blank control group, model group, dexamethasone intervention group, EEHDH aqueous extract group and alcohol extraction group.The dexamethasone intervention group, aqueous extract group and alcohol extraction group were continuously administered for 7 days, and the rest was given an equal volume of normal saline.Thirty minutes after the last administration, mice in the other groups were modeled by intraperitoneal injection of LPS (10 mg/kg) except for the blank control group.The blank group was given an equal volume of physiological saline every day during the experiment.Clinical symptoms were observed at 6 hours, 12 hours, 24 hours, and48 hours after the modeling.Serum inflammatory cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA).The histopathological changes in liver of mice were observed under light microscope.Result:Compared with the model group at the same time, the levels of serum IL-6, IL-1β and TNF-α in the mice treated with EEHDH aqueous extract and EEHDH alcohol extract were significantly decreased (P<0.05).In the comparison between groups, the above indicators of the 12 h node of EEHDH extract group were better than the 6 hnode, and compared with the EEHDH ethanol extract group, the EEHDH aqueous extract group at each time point Better results but no significant differences.Pathological results showed that the degree of hepatic injury in the mice of EEHDH extract group was significantly lighter than that of the model group, and the damage in the EEHDH extract group is even lighter.Conclusion:EEHDH extract can effectively improve liver injury in mice with LPS sepsis and inhibit the levels of IL-6, IL-1β and TNF-α.The mechanism may be to regulate the balance of the innate immune system by inhibiting the expression of this related inflammatory factor.