目的探讨黄芪总苷对大鼠退变颈椎间盘内白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量的影响。方法将110只SD大鼠随机分为空白对照组、模型组、黄芪总苷高剂量组、黄芪总苷中剂量组、黄芪总苷低剂量组和甲钴胺对照组,每组18只(2只造模失败剔除)。造模成功后,按要求给予药物灌胃,分别于灌胃前后监测大鼠的运动功能,灌胃1,2,4周后分3次处死大鼠,取各组大鼠颈椎间盘,用放射免疫分析法分别检测标本中IL-1β,TNF-α的含量。结果与空白对照组比较,造模成功后即给药前,其他5组斜板试验角度均明显减小(P<0.05);在给药1,2,4周后,与模型组比较,黄芪总苷高、中、低剂量组,甲钴胺对照组斜板试验角度均明显增大,IL-1β,TNF-α含量均明显降低(P<0.05),且以黄芪总苷中剂量组作用最为显著(P<0.05)。结论退变椎间盘不但释放大量的细胞因子,还可加速椎间盘的退变,黄芪总苷能降低退变颈椎间盘中细胞因子的含量,具有减弱炎性反应和减缓椎间盘退变的作用,且存在量效关系。 Objective To investigate the effects of total astragalosides on interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the degenerative cervical intervertebral disc in rats. Methods One hundred and thirteen Sprague-Dawley rats were randomly divided into blank control group, model group, high dose total astragaloside group, middle dose astragaloside group, low dose astragaloside group and control group, 18 rats in each group Only create failure to remove). After successful modeling, the rats were given gavage as required and the motor function of the rats was monitored before and after gavage. Rats were sacrificed three times after gavage for one, two and four weeks. The cervical intervertebral discs of each group were treated with radiation The contents of IL-1β and TNF-α were detected by immunoassay. Results Compared with the blank control group, the angles of the other five groups were significantly decreased (P <0.05) before the administration was successful, and after 1, 2 and 4 weeks of administration, compared with the model group, The concentrations of IL-1β and TNF-α in the group of high, medium and low dose of total glycosides and the group of mecobalamin control were significantly increased (P <0.05) The most significant (P <0.05). Conclusion Degenerative intervertebral disc not only release a large number of cytokines, but also accelerate the degeneration of intervertebral disc. Astragaloside can reduce the content of cytokines in degenerative cervical intervertebral disc, reduce the inflammatory reaction and degenerate the disc, Effective relationship.