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目的:研究高温处理补骨脂对酪氨酸酶活性的影响。方法:采用体外酪氨酸酶多巴速率氧化微量法,测定高温处理补骨脂后对酪氨酸酶活性的影响。结果:除低浓度和30 min中浓度外,同一浓度和高温处理时间下,随着温度的升高,对酪氨酸酶的激活率逐渐增大;同一浓度和温度下,随着高温处理时间的增大,对酪氨酸酶的激活率未呈现规律性的增强或减弱的趋势,其中,在2 g·L-1浓度下,121℃加热40 min和20 min对酪氨酸酶的激活率最高,分别为135.45%±4.29%和106.97%±3.21%。结论:控制合适的高温处理时间和温度可以增强补骨脂浸膏对酪氨酸酶的激活活性。
Objective: To study the effect of high temperature treatment of psoralen on tyrosinase activity. Methods: The in vitro tyrosinase dopa rate oxidation trace method was used to determine the effect of high temperature treatment of psoralen on tyrosinase activity. Results: Under the same concentration and high temperature treatment time, the activation rate of tyrosinase gradually increased with the increase of temperature except the low concentration and medium concentration of 30 min. Under the same concentration and temperature, with the increase of temperature, , The tyrosinase activation rate showed no regular increase or decrease trend. Among them, tyrosinase activation at 2 g · L-1, heating at 121 ℃ for 40 min and 20 min The highest rates were 135.45% ± 4.29% and 106.97% ± 3.21%, respectively. Conclusion: Controlling the appropriate time and temperature of high temperature can enhance the activation of tyrosinase by psoralen extract.