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目的:本文首次建立了用1-苯基-3-甲基-5-吡唑啉酮(PMP)作为柱前衍生化试剂对3-O-甲基-葡萄糖(3-OMG)进行衍生化的方法,并应用此方法对大鼠血浆中的3-OMG进行分析.方法:70℃水浴时,3-OMG在0.4 mol/L的NaOH条件下和PMP进行反应.衍生物采用反相高效液相色谱法进行分析,紫外检测波长为245 nm.结果:线性系数在0.991以上,最低定量限0.0078 mg/mL.回收率在98%~105%之间,且日间与日内变异不高于12%.本方法成功应用于糖尿病大鼠血浆中3-OMG的分析.结论:本方法简便、高效,适合对正常和糖尿病大鼠的血浆3-OMG进行分析.,AIM: To establish a convenient method to analyze 3-O-Methyl-D-Glucose (3-OMG) in rat plasma using 1-phenyl-3-methyl-5-pyrazolone (PMP) as a pre-column derivatizing agent, and in the mean time using this method to analyze the 3-OMG in rat plasma. METHODS:3-OMG reacted easily with FMP in the presence of 0. 4 mol/L NaOH at a 70 ℃water bath. The derivatives were analyzed by Reverse Phase High Performance Liquid Chromatography ( RP-HPLC) and monitored by ultraviolet detector at 245 nm. RESULTS: All linear coefficients were o-ver 0. 991, and the quantitative limit was 0. 0078 mg/mL. The recoveries were found to be 98% -105%. Variations of intra- and inter-day preci-sion were less than 12%. The method was suc-cessfully applied to the analysis of 3-OMG in rat plasma. CONCLUSION: This method is simple and effective, and can be adopted to analyze the 3-OMG in the plasma from both normal and dia-betic rats.