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目的 :观察急性白血病化疗后“凋亡彗星”的变化及其与临床疗效的关系。方法 :采用改良 Singh法 (改良碱性单细胞凝胶电泳 ,即“彗星试验”)检测 15例急性白血病患者化疗后 4、8、12、2 4、36和 72 h“凋亡彗星”的变化。结果 :化疗前“凋亡彗星”均 <0 .5 % ,化疗 8h开始增高 ,2 4h达高峰 ,“凋亡彗星”百分率 >98.0 %、骨髓白血病细胞减少指数 (MBDI) >6 5 .0 %的 11例经 1个疗程化疗完全缓解 ;“凋亡彗星”百分率为 95 .5 %、MBDI为18.5 %的 1例经 2个疗程化疗完全缓解 ;“凋亡彗星”百分率为 84.0 %、MBDI为 6 .1%的 1例经 2个疗程化疗部分缓解 ;“凋亡彗星”百分率为 3.0 %和 5 .5 %、MBDI为 1.1%和 1.6 %的 2例患者临床未缓解。结论 :应用“彗星试验”在急性白血病患者化疗过程中可检出大量早期凋亡细胞 ;“凋亡彗星”的检测是早期判断化疗疗效的良好指标。
Objective: To observe the change of “apoptosis comet” after acute leukemia chemotherapy and its relationship with clinical curative effect. Methods: The changes of “apoptosis comets” in 15 acute leukemia patients at 4, 8, 12, 24, 36 and 72 h after chemotherapy were detected by modified Singh method (modified alkaline single-cell gel electrophoresis . Results: Before chemotherapy, all the apoptotic comets were less than 0.5%. The levels of apoptosis were higher at 8 hours and reached the peak at 24 hours. The percentage of apoptotic comets was> 98.0% and the MBDI was 65.5% Of the 11 cases of complete remission after a course of chemotherapy; “apoptotic comet” percentage was 95.5%, MBDI was 18.5% in 1 case of 2 courses of chemotherapy complete remission; “apoptotic comet” percentage was 84.0% MBDI 6.1% of the patients were partly relieved after 2 courses of chemotherapy; 2 patients with 3.0% and 5.5% of apoptotic comets and 2% of MBDI were 1.1% and 1.6% respectively. CONCLUSION: A large number of early apoptotic cells can be detected during the chemotherapy of patients with acute leukemia using “comet assay”. The detection of “comet apoptosis” is a good indicator of early response to chemotherapy.