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利用在300余份来源于辽宁、吉林、黑龙江、内蒙古、江苏等地的杂草稻材料中筛选出耐高盐杂草稻材料WR03-12。通过RT-PCR的方法得到盐胁迫下WR03-12与盐敏感栽培稻‘越光’幼苗的cDNA第一链,对3个锌指蛋白基因家族的6个基因表达情况进行了荧光实时定量分析。结果表明,2个C2C2型锌指蛋白基因SRZ1与SRZ2受到高盐胁迫的负向诱导,WR03-12受负向诱导程度要小于‘越光’;2个TFIIIA型锌指蛋白基因ZFP18与ZFP245受到盐胁迫的正向诱导,WR03-12受诱导程度也小于‘越光’;具有A20锌指结构的基因AACZ1基因在越光中不受盐诱导,而在WR03-12中受短时间诱导后,第7天已经恢复到胁迫前水平。具有AN1锌指结构的基因AACZ2在‘越光’与WR03-12中均不受盐胁迫诱导,且表达水平没有显著差别。杂草稻WR03-12与‘越光’对于盐胁迫的应答机制可能在转录调控方面存在差别。
Using more than 300 copies of weedy rice materials from Liaoning, Jilin, Heilongjiang, Inner Mongolia and Jiangsu, we screened WR03-12, a high-salt weedy rice material. The first strand cDNA of WR03-12 and salt sensitive rice ’Koshihikari’ seedlings under salt stress was obtained by RT-PCR, and the real-time quantitative analysis of 6 genes expression in three zinc finger protein gene families was performed. The results showed that two of the C2C2 zinc finger protein genes SRZ1 and SRZ2 were negatively induced by high salt stress and WR03-12 was less negative than ’Koshihikari’; two TFIIIA zinc finger protein genes ZFP18 and ZFP245 were affected Under the salt stress, WR03-12 was also less induced than that of ’Koshihikari’. The AACZ1 gene with A20 zinc finger was not induced by salt in Koshihikari, but was induced by WR03-12 for a short time. The first 7 days have returned to the pre-stress level. The gene AACZ2 with AN1 zinc finger structure was not induced by salt stress in both ’Koshihikari’ and WR03-12, and the expression level was not significantly different. The response mechanisms of weedy rice WR03-12 and ’Koshihikari’ to salt stress may be different in transcriptional regulation.