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采用胶原酶消化法分离培养人大网膜前脂肪细胞。曲格列酮干预细胞,油红O染色鉴定分化状态,ELISA测定脂肪细胞因子分泌水平,RT-PCR观察分化转录因子和脂肪细胞因子mRNA表达,以期从内脏脂肪的储脂和内分泌功能角度探讨噻唑烷二酮类药物的作用机制。结果发现,曲格列酮能促进人前脂肪细胞分化,提高脂肪细胞储脂能力;显著增加脂联素分泌;调节瘦素分泌;抑制抵抗素分泌,提示噻唑烷二酮类药物可通过调节脂肪细胞因子谱改善机体胰岛素抵抗和炎症状态。其作用机制可能主要通过促进PPARγ2、C/EBPα和脂蛋白酯酶(Lipoprotein lipase,LPL)等基因的表达。脂肪细胞的增加和瘦素分泌的改变可能部分解释噻唑烷二酮类药物增加体重的负面效应。
Isolation and culture of human omental preadipocytes with collagenase digestion. Troglitazone intervention cells, oil red O staining to identify differentiation status, ELISA measurement of adipocytokines level, RT-PCR observation of differentiation transcription factors and adipocytokines mRNA expression in visceral fat from the fat storage and endocrine function of the perspective of thiazole Mechanism of action of diketones. The results showed that troglitazone could promote the differentiation of human preadipocytes and increase the fat storage ability of adipocytes, significantly increase adiponectin secretion, regulate leptin secretion and inhibit the secretion of resistin, suggesting that thiazolidinedione can regulate adipocytes Factor spectrum to improve the body’s insulin resistance and inflammatory status. Its mechanism may be mainly through the promotion of PPARγ2, C / EBPα and lipoprotein lipase (LPL) and other genes. Increased adipocytes and changes in leptin secretion may partially explain the negative effects of thiazolidinediones on weight gain.