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从人心肌组织中提纯了酸性铁蛋白,免疫家兔产生抗血清,氯胺-T法制备了~(125)I-酸性铁蛋白和~(125)I-抗酸性铁蛋白H亚基单抗。分别采用平衡法和顺序饱和法建立了酸性铁蛋白放射免疫分析和酸性铁蛋白H亚基免疫放射测量分析。它们的批内CV分别为1.65％和2.44％,批间CV为9.71％和10.16％,回收率为102％和92.29％,ED_(50)为27.50μg/L和23.05μg/L。前者的可测范围为7.0～369.6μg/L,后者的灵敏度为0.736μg/L。两方法
Purification of acidic ferritin from human myocardial tissue, immunization of rabbits to produce antiserum, and preparation of 125 I-acid ferritin and 125I-anti-acid ferritin H subunit mAb by chloramine-T method . The radio-immunoassay of acid ferritin and the immunoradiometric analysis of acid ferritin H subunit were established using the equilibrium method and sequential saturation method, respectively. Their intra-assay CVs were 1.65% and 2.44%, inter-assay CVs were 9.71% and 10.16%, and the recoveries were 102% and 92.29%, respectively. ED_(50) was 27.50 μg/L and 23.05 μg/L. The former has a measurable range of 7.0-369.6 μg/L and the latter has a sensitivity of 0.736 μg/L. Two methods