论文部分内容阅读
AIM To investigate the influence of L-methionine-deprived total parenteral nutrition with 5-FU on gastriccancer and host metabolism.METHODS N-methyI-N’-nitro-nitrosoguanidine(MNNG)induced gastric cancer rats were randomly divided intofour groups:Met-containing TPN group(n=11),Met-deprived TPN group(n=12),Met-containing TPN+5-FUgroup(n=11)and Met-deprived TPN+5-FU group(n=12).Five rats in each group were sacrificed after 7days of treatment and the samples were taken forexamination.The remaining rats in each group were thenfed separately with normal diet after the treatment untildeath,the life span was noted.RESULTS The tumors were enlarged in Met-containinggroup and shrank in Met-deprived group markedly after thetreatment.The DNA index(DI)of tumor cells and the bodyweight(BW)of rats had no significant change in the twogroups,however,the ratio of tumor cells S phase wasincreased.The ratio of G2M phase went up in Met-containing group,but down in Met-deprived group.In theother two groups that 5-FU was added,the BW of rats,and the diameter of tumors,the DI of tumor cells,the Sand G2M phase ratio of tumor cells were all decreased,particularly in Met-deprived plus 5-FU group.Pathologicalexamination revealed that the necrotic foci of the tumortissue increased after Met-deprived TPN treatment,andthe nucleoli of tumor cells enlarged.In-MetTPN+5-FUgroup,severe nuclear damage was also found bykaryopyknosis and karyorrhexis,meanwhile there wasslight degeneration in some liver and kidney cells.Theserum free Met and Cysteine decreased markedly(P<0.001),while other amino acids,such as serum freeserine and glutamine increased significantly(P<0.005).All the rats died of multiple organ failure caused by cancermetastasis.The average survival time was 18.6 days inMet-containing TPN group,31 days in Met-deprived TPNgroup,27.5 days in Met-containing TPN+5-FU group,and43 days in Met-deprived TPN+5-FU group(P<0.05). CONCLUSION Met-deprived TPN causes methioninestarvation of tumor cells,and can enhance the anti-tumoreffect of 5-FU and prolong the life span of gastric cancer-bearing rats.
AIM To investigate the influence of L-methionine-deprived total parenteral nutrition with 5-FU on gastriccancer and host metabolism.METHODS N-methyI-N’-nitro-nitrosoguanidine(MNNG)induced gastric cancer rats were randomly divided into four groups:Met- Containing TPN group (n=11), Met-deprived TPN group (n=12), Met-containing TPN+5-FUgroup (n=11) and Met-deprived TPN+5-FU group (n=12).Five Rats in each group were sacrificed after 7days of treatment and the samples were taken forexamination. The remaining rats in each group were thenfed separately with normal diet after the treatment untildeath, the life span was noted.RESULTS The tumors were enlarged in Met-containinggroup and Shrank in Met-deprived group markedly after the treatment.The DNA index(DI)of tumor cells and the bodyweight(BW)of rats had no significant change in the twogroups,however,the ratio of tumor cells S phase wasincreased.The ratio of G2M Phase went up in Met-containing group,but down in Met-deprived group.In the other two groups t Hat 5-FU was added, the BW of rats, and the diameter of tumors, the DI of tumor cells, the Sand G2M phase ratio of tumor cells were all decreased, specifically in Met-deprived plus 5-FU group.Pathologicalexaminationwn that The necrotic foci of the tumortissue increased after Met-deprived TPN treatment,andthe nucleoli of tumor cells enlarged.In-MetTPN+5-FUgroup,severe nuclear damage was also found bykaryopyknosis and karyorrhexis,mewhiwhire there wasslight degeneration in some liver and kidney cells. Theserum free Met and Cysteine was marked markedly (P<0.001), while other amino acids,such as serum freeserine and glutamine increased significantly(P<0.005).All the rats died of multiple organ failure caused by cancermetastasis.The average survival time was 18.6 Days inMet-containing TPN group, 31 days in Met-deprived TPNgroup, 27.5 days in Met-containing TPN+5-FU group, and43 days in Met-deprived TPN+5-FU group (P<0.05). CONCLUSION Met-deprived TPN causes methioninestarvation of tumor cells,andCan enhance the anti-tumoreffect of 5-FU and prolong the life span of gastric cancer-bearing rats.