[Objective] This study was to investigate the distribution of H-FABP for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig×Sus scrofa. [Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, longissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.