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目的研究妊娠期高血压疾病患者静脉血清及胎儿脐血中巨噬细胞移动抑制因子(MIF)和高迁移率蛋白-1(HMGB1)的变化及意义。方法采用酶联免疫吸附法(ELISA)进行MIF和HMGB1含量的测定,分别测定90例妊娠期高血压疾病患者(其中妊娠期高血压、轻度子痫前期、重度子痫前期各30例)及30例正常对照组产妇静脉血清及胎儿脐血中MIF和HMGB1的含量。结果 MIF和HMGB1在妊娠期高血压疾病组及对照组血清及胎儿脐血中均有表达。MIF在妊娠期高血压疾病各组血清中含量与对照组血清中的比较,差异有统计学意义(t=7.31,9.82,10.04,P值均<0.05),且重度子痫前期组明显高于轻度子痫前期和妊娠期高血压组(t=7.23,8.95,P值均<0.05);轻度子痫前期组明显高于妊娠期高血压组(t=7.68,P<0.05)。HMGB1在妊娠期高血压疾病各组血清中含量与对照组血清中的比较,差异有统计学意义(t=7.54,9.65,10.18,P值均<0.05),且重度子痫前期组明显高于轻度子痫前期和妊娠期高血压组(t=7.54,9.65,P值均<0.05);轻度子痫前期组明显高于妊娠期高血压组(t=7.35,P<0.05)。妊娠期高血压疾病患者MIF与HMGB1含量呈正相关(r=0.59,P<0.01)。MIF和HMGB1在妊娠期高血压疾病各组胎儿脐血中的含量,差异无统计学意义(P>0.05)。结论 MIF和HMGB1的高表达说明,妊娠期高血压疾病患者内皮细胞损伤,且MIF和HMGB1在损伤过程中起作用。
Objective To study the changes of macrophage migration inhibitory factor (MIF) and high mobility group-1 (HMGB1) in venous and fetal umbilical blood in patients with gestational hypertension. Methods The levels of MIF and HMGB1 were determined by enzyme-linked immunosorbent assay (ELISA). The levels of MIF and HMGB1 were measured in 90 patients with gestational hypertension (gestational hypertension, mild preeclampsia and severe preeclampsia, respectively) and 30 cases of normal control group of maternal venous serum and fetal cord blood MIF and HMGB1 content. Results Both MIF and HMGB1 were expressed in serum and fetal umbilical cord blood of patients with gestational hypertension and controls. The serum levels of MIF in each group of hypertensive disorder complicating pregnancy were significantly lower than those in the control group (t = 7.31,9.82,10.04, P <0.05), and were significantly higher than those in severe preeclampsia Mild preeclampsia and gestational hypertension group (t = 7.23, 8.95, P <0.05). Mild preeclampsia group was significantly higher than gestational hypertension group (t = 7.68, P <0.05). The serum levels of HMGB1 in each group of hypertensive disorder complicating pregnancy were significantly higher than those in the control group (t = 7.54,9.65,10.18, P <0.05), and were significantly higher than those in severe preeclampsia Mild preeclampsia and gestational hypertension group (t = 7.54,9.65, P <0.05). Mild preeclampsia group was significantly higher than gestational hypertension group (t = 7.35, P <0.05). The levels of MIF and HMGB1 in patients with gestational hypertension were positively correlated (r = 0.59, P <0.01). The levels of MIF and HMGB1 in umbilical blood of fetuses with gestational hypertension were not significantly different (P> 0.05). Conclusion The high expression of MIF and HMGB1 indicates that endothelial cells are impaired in patients with hypertensive disorders of pregnancy, and MIF and HMGB1 play a role in the process of injury.