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目的探讨长时间睡眠剥夺（sleepdeprivation，SD）对大鼠下丘脑和脑干5-羟色胺（5-HT）代谢及行为的影响。方法用水上转盘（dish-over-water）睡眠剥夺模型对大鼠分别进行24h、48h、72h睡眠剥夺，观察睡眠剥夺后下丘脑、脑干两脑区5-HT代谢的变化，同时利用旷场试验观察大鼠主动行为的改变。结果睡眠剥夺大鼠的旷场试验得分较非剥夺大鼠高（P＜0.05），但随着时间的延长出现下降趋势;5-HT向5-羟吲哚乙酸（5-HIAA）转化在经过24h睡眠剥夺后显著增高（P＜0.01），但在48h开始下降，72h后出现大幅下降（P＜0.01）。结论长时间睡眠剥夺对大鼠行为的影响是一个由兴奋到抑制的过程，这种变化可能与下丘脑和脑干的5-HT代谢有关。
Objective To investigate the effects of prolonged sleep deprivation (SD) on the metabolism and behavior of 5-HT in rat hypothalamus and brain stem. Methods The rats were deprived of sleep for 24 h, 48 h and 72 h respectively by using sleep-deprivation model with dish-over-water. The changes of 5-HT metabolism in hypothalamus and brainstem after sleep deprivation were observed. Experiment to observe the change of active behavior in rats. Results The scores of open-field test in sleep-deprived rats were higher than those in non-deprived rats (P <0.05), but decreased with time. The 5-HT to 5-HIAA 24h sleep deprivation was significantly higher (P <0.01), but began to decline in 48h, 72h after a significant decline (P <0.01). Conclusion The effect of prolonged sleep deprivation on the behavior of rats is a process from excitement to inhibition, which may be related to 5-HT metabolism in the hypothalamus and brain stem.