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目的探讨睾丸特异性Y样蛋白5(TSPYL5)表达对胃癌生物学行为的影响。方法采用RT-PCR方法检测36例胃癌组织、癌旁正常胃黏膜组织中TSPYL5mRNA的表达,采用细胞划痕实验研究TSPYL5对胃癌细胞株AGS迁移能力的影响。结果 36例中,25例(69.4%)胃癌组织中TSPYL5mRNA表达显著下调。TNMⅡ、Ⅲ期以及无腹腔和远处转移者TSPYL5mRNA表达明显下调(P<0.05)。体外实验显示,TSPYL5对AGS细胞迁移的抑制作用呈浓度依赖性加强。结论 TSPYL5mRNA表达下调可能与胃癌的发生发展相关。
Objective To investigate the effect of testicular specific Y-like protein 5 (TSPYL5) expression on the biological behavior of gastric cancer. Methods TSPYL5 mRNA expression was detected by RT-PCR in 36 gastric cancer tissues and adjacent normal gastric mucosa tissues. Cell scratch assay was used to study the effect of TSPYL5 on the migration of AGS in gastric cancer cell lines. Results Among the 36 cases, TSPYL5 mRNA expression was significantly down-regulated in 25 cases (69.4%) of gastric cancer tissues. The expression of TSPYL5mRNA in TNMⅡ, Ⅲphase and without metastasis to abdominal cavity and distant place were significantly decreased (P <0.05). In vitro experiments showed that TSPYL5 inhibited AGS cell migration in a concentration-dependent manner. Conclusion The down-regulation of TSPYL5 mRNA may be related to the occurrence and development of gastric cancer.