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目的研究贵州产五步蛇蛇毒磷脂酶Ａ２的抗凝血及抗血栓作用。方法对大鼠静脉注射不同剂量的ＰＬＡ２，测定大鼠的凝血时间、凝血酶原时间、抗凝效价和血栓抑制率。结果ＰＬＡ２浓度达到１．０ｍｇ／ｋｇ以上，能显著延长大鼠凝血时间及凝血酶原时间，其抗凝效价相当于肝素钠的２４．８％～３１．５％。ＰＬＡ２能显著抑制大鼠实验性动脉及静脉血栓的形成。结论贵州产五步蛇ＰＬＡ２具有较强的抗凝血和抗血栓作用。
Objective To study the anticoagulant and antithrombotic effects of Phospholipase A2 in Wudu snake venom from Guizhou. Methods Different doses of PLA2 were intravenously injected into rats, and the clotting time, prothrombin time, anticoagulant titer and thrombus inhibition rate were determined. Results PLA2 concentration of 1.0mg / kg or more, can significantly extend the clotting time and prothrombin time in rats, the anticoagulant titer equivalent to heparin sodium 24.8% to 31.5%. PLA2 can significantly inhibit the formation of experimental arterial and venous thrombosis in rats. CONCLUSION PLA2 produced by Guizhou five-step snake has strong anticoagulant and antithrombotic effects.