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机体在抵抗结核杆菌时,T细胞免疫起着重要作用。病程达3年以上的活动性肺结核患者周围血淋巴细胞(PBL)的增殖反应减弱,影响了病灶的愈合。这种反应可通过PBL在体外对纯蛋白衍生物(PPD)的应答状况加以检测。将参加试验的46例活动性肺结核患者分为新病例组和难治性晚期病例组,每组23人,健康对照组32人。将受试者的PBL与PPD于37℃共育6天,收获细胞前18小时加入[~3H]胸苷,用闪烁计数器测定每组细胞的掺入放射性,最后以平均计数/分(cpm)表示。计数结果是,晚期病例组为20.0±10.8×10~3,新病例组30.5±10.5×10~3,前组的增
When the body is resistant to Mycobacterium tuberculosis, T cell immunity plays an important role. The proliferative response of peripheral blood lymphocytes (PBLs) in patients with active pulmonary tuberculosis who have been active for more than 3 years is weakened, affecting the healing of the lesion. This response can be detected by PBL in vitro response to pure protein derivatives (PPD). The 46 active tuberculosis patients participating in the trial were divided into a new case group and a refractory advanced case group, with 23 in each group and 32 in the healthy control group. The PBL and PPD of the subject were co-cultured for 6 days at 37 ° C, [~ 3H] thymidine was added 18 hours prior to harvest, radioactivity incorporation in each group of cells was measured by a scintillation counter, and the average counts / minute Said. Counting results, the late case group was 20.0 ± 10.8 × 10 ~ 3, the new case group 30.5 ± 10.5 × 10 ~ 3, the former group by