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目的了解天津地区儿童急性上呼吸道感染中病毒分布情况,探讨不同病毒的流行趋势,为临床诊断与治疗提供病原学依据,同时为今后制定正确的预防策略奠定基础。方法采用多重RT-PCR法,对249份咽拭子标本同时检测腺病毒,人偏肺病毒,人冠状病毒229E/NL63,1型、2型、3型副流感病毒,甲型、乙型流感病毒,甲型、乙型呼吸道合胞病毒,A/B型人鼻病毒,人冠状病毒OC43/HKU1共12种常见呼吸道病毒。结果 249份标本中共检出阳性标本144份,阳性率为57.83%,2种以上病毒混合感染为7份,占2.81%;在阳性检测结果中流感病毒占53.64%,腺病毒占21.19%,呼吸道合胞病毒占8.61%;流感病毒中H3N2亚型占49.38%,甲型H1N1亚型占34.57%,B型占16.05%。结论流感病毒、腺病毒和呼吸道合胞病毒为天津地区儿童急性上呼吸道病毒感染的主要病原体,不同的病毒在时间上有一定的流行规律。
Objective To understand the distribution of the virus in children with acute upper respiratory tract infection in Tianjin and to explore the epidemiological trends of different viruses so as to provide etiological basis for clinical diagnosis and treatment and to lay the foundation for the formulation of correct prevention strategies in the future. Methods A total of 249 throat swab specimens were tested for simultaneous detection of adenovirus, human metapneumovirus, human coronavirus 229E / NL63, type 1, type 2 and type 3 parainfluenza virus, type A and type B influenza virus by multiplex RT-PCR. Viruses, A, B respiratory syncytial virus, A / B type human rhinovirus, human coronavirus OC43 / HKU1 a total of 12 kinds of common respiratory viruses. Results A total of 144 positive samples were detected in 249 samples, the positive rate was 57.83%. The mixed infection rates of 2 or more viruses were 7, accounting for 2.81%. Among the positive results, 53.64% were influenza viruses, 21.19% were adenoviruses, Syncytial virus accounted for 8.61%; influenza virus H3N2 subtype accounted for 49.38%, type A H1N1 subtype 34.57%, type B accounted for 16.05%. Conclusion Influenza virus, adenovirus and respiratory syncytial virus are the main pathogens of acute upper respiratory tract infection in children in Tianjin. Different viruses have certain epidemic patterns in time.