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免疫组化技术对病理诊断的作用越来越为大家共识。但常规的福尔马林固定石蜡包埋组织有许多抗原被掩盖。雌激素（ER）和孕激素（PR）的状态与乳腺癌治疗密切相关，是判断预后的重要参数，而其免疫组化的定位是比较难的，人们相继采取各种酶、微波和高压锅处理都得到阳性的不同
The role of immunohistochemistry in pathological diagnosis is becoming more and more common. However, many formalin-fixed paraffin-embedded tissues have many antigens masked. The status of estrogen (ER) and progesterone (PR) is closely related to the treatment of breast cancer and is an important parameter to judge the prognosis. However, the localization of ER and PR is more difficult. People have successively taken various enzymes, microwaves and autoclaves Have been positive different