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目的:比较microRNA-106b(miR-106b)在肾癌及癌旁正常组织之间和肾癌细胞系和正常肾细胞系之间的表达水平,并分析其表达水平与临床病理资料之间的关系。方法:采用RT-qPCR方法检测miR-106b表达量在35对肾癌组织和癌旁正常组织中的差异,并运用秩和检验方法分析肾癌组织中miR-106b表达量与患者临床病理特征之间的相关性。培养肾癌细胞系(786-O和ACHN)和正常肾上皮细胞系(HK-2)并采用RT-qPCR方法检测细胞miR-106b表达量。结果:miR-106b在肾癌组织中的表达量明显高于癌旁正常组织(P<0.01),且肾癌组织中miR-106b表达量与肾癌的组织类型有相关性(P=0.049),未发现与其他临床病理资料如年龄、性别、T分期、Fuhrman分级和AJCC临床分期有明显相关关系(P>0.05)。同时,miR-106b表达量在肾癌细胞系中也明显高于正常肾上皮细胞系(P<0.01)。结论:miR-106b在肾癌组织和细胞系中均高表达,且其在肾癌组织中的表达水平与肾癌组织类型相关,提示miR-106b与肾癌的发生和发展相关。
OBJECTIVE: To compare the expression of microRNA-106b (miR-106b) between renal cell carcinoma and adjacent normal tissues and between renal cell carcinoma cell lines and normal renal cell lines, and analyze the relationship between the expression of miR-106b and clinicopathological data . Methods: The expression of miR-106b was detected by RT-qPCR in 35 pairs of normal renal tissues and adjacent normal tissues. The rank sum test was used to analyze the expression of miR-106b in renal cell carcinoma and the clinicopathological features Relevance between. The renal cancer cell lines (786-O and ACHN) and normal renal epithelial cell line (HK-2) were cultured and the expression of miR-106b was detected by RT-qPCR. Results: The expression of miR-106b in renal cell carcinoma was significantly higher than that in adjacent normal tissues (P <0.01), and the expression of miR-106b in renal cell carcinoma was correlated with the type of renal cell carcinoma (P = 0.049) No significant correlations were found with other clinical and pathological data such as age, sex, T stage, Fuhrman grade and clinical stage of AJCC (P> 0.05). Meanwhile, the expression of miR-106b was also significantly higher in renal cell carcinoma than in normal renal epithelial cells (P <0.01). Conclusion: miR-106b is highly expressed in both renal cell carcinoma and cell lines, and its expression in renal cell carcinoma is related to the type of renal cell carcinoma, suggesting that miR-106b is associated with the occurrence and development of renal cell carcinoma.