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以豚鼠肺巨噬细胞为研究对象，研究了甲醛和苯并（ａ）芘（ＢａＰ）在ＬＤ５０、ＤＮＡ－蛋白质交链（ＤＰＣ）、ＤＮＡ单链断裂（ＳＳＢ）等３项指标上的联合作用。结果表明，甲醛和苯并（ａ）芘对豚鼠肺巨噬细胞的ＬＤ５０分别为２４０．４ｍｇ／Ｌ和１３．８ｍｇ／Ｌ，联合使用的ＬＤ５０为６１．７ｍｇ／Ｌ（其中甲醛为５５．８ｍｇ／Ｌ，ＢａＰ为５．８８ｍｇ／Ｌ），呈增强作用。甲醛能引起ＤＰＣ，ＢａＰ不能引起ＤＰＣ，联合作用时甲醛引起的交链程度降低；甲醛和ＢａＰ都能引起ＳＳＢ，联合作用时ＳＳＢ的程度增强。由此可见，甲醛和ＢａＰ对细胞杀伤和ＳＳＢ具有增毒作用。而ＤＰＣ受到抑制
In this study, guinea pig lung macrophages were used to study the combined effects of formaldehyde and benzo (a) pyrene (BaP) on three indexes of LD50, DPC and SSB . The results showed that the LD50 of formaldehyde and benzo (a) pyrene on guinea pig lung macrophages were 240.4 mg / L and 13.8 mg / L, respectively. The combined LD50 was 61.7 mg / L (formaldehyde was 55.8 mg / L, BaP 5.88mg / L), was enhanced. Formaldehyde can cause DPC, BaP can not cause DPC, the combined effect of formaldehyde caused less degree of cross-linked; Formaldehyde and BaP can cause SSB, the combined effect of SSB increased. Thus, formaldehyde and BaP on cell killing and SSB has an increased toxic effect. DPC is suppressed