根据已知的黄瓜花叶病毒,百合无症病毒、百合斑驳病毒基因核苷酸序列,设计引物和探针,制备寡核苷酸芯片。用Cy3标记核苷酸引物,不对称RT-PCR扩增产物与芯片上的寡核苷酸探针杂交,荧光扫描仪检测并分析信号。研究制备的基因芯片能够检测侵染百合的3种重要病毒核酸的特异性荧光信号,该项技术具有特异、灵敏、快速的优点。 Based on the known cucumber mosaic virus, lily etiology virus, lily mottle virus gene nucleotide sequence, primers and probes were designed to prepare oligonucleotide chip. Nucleotide primers were labeled with Cy3, and asymmetric RT-PCR amplification products were hybridized with on-chip oligonucleotide probes. Fluorescent scanners were used to detect and analyze the signals. The gene chip prepared by this study can detect the specific fluorescence signal of the three important viral nucleic acids that infects lily, which has the advantages of specificity, sensitivity and rapidity.