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92只Lewis鼠,按20毫克/公斤体重,静注二甲基亚硝胺(DMNA),造成急性肝衰竭。24小时后,分成4组按下述治疗:第Ⅰ组(19只):腹膜内移植2.0克正常同系鼠肝细胞;第Ⅱ组(24只):门静脉灌注1.5克正常同系鼠肝细胞;第Ⅲ组(18只):门静脉灌注盐水;第Ⅳ组(31只):不予治疗。结果:注射DMNA后3天,各组血清谷草转氨酶平均值皆相似,表明各组肝损害程度大致相同,但在注射DMNA后3周,Ⅰ、Ⅱ(63％和71％)组存活率明显高于Ⅲ、Ⅳ组(17％和6％),说明不论是腹膜移植还是经门静脉移植分散的肝细胞,均能提供充分的代谢支持,以使药物诱发的肝坏死得以恢复(存活的动物,约在3周左右,肝结构恢复至接近正常)。注射DMNA后第3、5天,各组血清胆红素浓度皆升高,但Ⅰ、Ⅱ组平均值明显低于Ⅲ、Ⅳ组
Ninety-two Lewis rats were dosed intravenously with dimethylnitrosamine (DMNA) at 20 mg / kg body weight to cause acute liver failure. Twenty four hours later, the mice were divided into four groups according to the following treatment: Group I (19): intraperitoneal transplantation of 2.0 g of normal syngeneic rat hepatocytes; Group II (24): portal vein perfusion of 1.5 g of normal syngeneic rat hepatocytes; Group Ⅲ (n = 18): portal vein perfusion of saline; group Ⅳ (n = 31): no treatment. Results: The average serum aspartate aminotransferase levels in each group were similar at 3 days after injection of DMNA, indicating that the degree of liver damage in each group was about the same, but the survival rates in groups I and II (63% and 71%) were significantly higher at 3 weeks after DMNA injection In groups III and IV (17% and 6%), all hepatocytes, either peritoneal or portal vein-transplanted, provided adequate metabolic support for the recovery of drug-induced liver necrosis (viable animals, approximately At about 3 weeks, the liver structure returned to near normal). On the 3rd and 5th days after injection of DMNA, the serum bilirubin concentrations increased in all groups, but the average values in group Ⅰ and Ⅱ were significantly lower than those in groups Ⅲ and Ⅳ