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为了阐明草地螟Loxostege sticticalis大发生种群幼虫取食行为特征,在室内条件下(温度22±1℃,相对湿度70%)对不同幼虫密度[1,10,30头/瓶(650mL)]饲养草地螟幼虫的食物利用率及消化酶活性进行了研究。结果表明:幼虫中等(或高)密度对草地螟幼虫相对中肠重量、相对取食量、粪便干重、食物利用率和近似消化率及总蛋白酶和亮氨酸氨肽酶活性影响显著。幼虫相对中肠重量以10头/瓶的幼虫密度最大,1头/瓶的幼虫密度最小。随着幼虫密度的增加,幼虫相对取食量和粪便干重增加,而虫体干重减轻,幼虫食物利用率降低。幼虫密度30头/瓶的幼虫相对取食量和粪便干重显著高于1和10头/瓶的,而30头/瓶的幼虫食物利用率显著低于1头/瓶的。幼虫近似消化率随幼虫密度的逐渐增加而显著降低。幼虫密度10头/瓶的幼虫总蛋白酶和亮氨酸氨肽酶的活性显著高于1和30头/瓶的,而淀粉酶的活性受幼虫密度影响不显著。随幼虫密度的增加,幼虫相对中肠重量与总蛋白酶和亮氨酸氨肽酶活性变化趋势较为一致,消化酶活性的变化可能与相对中肠重量大小有关。因此,幼虫密度是影响草地螟幼虫取食行为的重要因子,这些结果为阐明草地螟大发生种群与一般种群的为害特征提供了重要理论依据。
In order to elucidate the larval feeding behavior of Loxostege sticticalis, the larval density (1, 10, 30 / bottle (650 mL)] was fed on grassland under indoor conditions (temperature 22 ± 1 ℃ and relative humidity 70% The food utilization rate and digestive enzyme activity of borer larvae were studied. The results showed that the medium (or high) density of larvae significantly affected the relative midgut weight, relative food intake, excrement dry weight, food utilization and approximate digestibility, and total protease and leucine aminopeptidase activity of larvae of the larvae. Larval relative midgut weight to 10 / bottle larval density maximum, 1 / bottle larval density minimum. With the increase of larval density, larvae relative feeding amount and excrement dry weight increased, while dry weight of parasites decreased, larvae food utilization rate decreased. The larval relative feeding and stool dry weight of larvae with a density of 30 heads / bottle were significantly higher than those of 1 and 10 heads per bottle, while the food utilization rate of 30 larvae / bottle was significantly lower than that of 1 per bottle. The approximate digestibility of larvae was significantly decreased with the gradual increase of larval density. The larval total protease and leucine aminopeptidase activities of larvae with a density of 10 heads / bottle were significantly higher than those of 1 and 30 heads / bottle, while the amylase activity was not significantly affected by larval density. With the increase of larval density, the relative midgut weight of larvae was consistent with that of total protease and leucine aminopeptidase. The change of digestive enzyme activity might be related to the relative weight of midgut. Therefore, the density of larvae is an important factor affecting the feeding behavior of the larvae of the meadow fester, and these results provide important theoretical basis for elucidating the damage characteristics of the large population and the general population of the larvae.