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[目的]以尿中多环芳烃代谢产物2-羟基萘(2-NAP)、2-羟基芴(2-FLU)、9-羟基菲(9-PHE)和1-羟基芘(1-OHP)作为生物标志,评价焦化厂工人体内多环芳烃(PAHs)暴露水平,探讨4种PAHs代谢物浓度的影响因素。[方法]对某焦化厂焦炉工437人(接触组)及非焦炉工167人(对照组)进行问卷调查,高效液相色谱-荧光检测法测定两组工人晨尿中4种多环芳烃代谢物水平。[结果]接触组尿中羟基代谢产物2-NAP、2-FLU、9-PHE和1-OHP浓度的中位数[分别为0.725、0.300、0.092、0.059μg/mmo L(以Cr计)]均显著高于对照组[分别为0.615、0.197、0.065、0.040μg/mmo L(以Cr计)](P<0.01);逐步logistic回归分析显示吸烟量每增加一个等级,致2-NAP发生高浓度的可能性增加了3.393倍;接触组的代谢物2-NAP、2-FLU、9-PHE和1-OHP发生高浓度的可能性分别是对照组的1.665、3.399、2.185和2.905倍。[结论]影响人群尿中多环芳烃代谢物浓度的主要因素是工种和吸烟,其中2-FLU与1-OHP是焦炉工职业暴露敏感的生物标志,而2-NAP是吸烟暴露敏感的生物标志。
[Objective] To investigate the effects of 2-Naphthalene (2-NAP), 2-FLU, 9-PHE and 1-OHP on the metabolism of PAHs in urine. As a biomarker, the level of PAHs exposure in coking plant workers was evaluated and the influencing factors of four PAHs metabolite concentrations were explored. [Method] A total of 437 coke oven workers (contact group) and 167 non-coke oven workers (control group) in a coking plant were surveyed by questionnaire. Four polycycles in morning urine of workers in two groups were measured by high performance liquid chromatography-fluorescence detection Aromatic metabolite levels. [Results] The median urinary metabolites 2-NAP, 2-FLU, 9-PHE and 1-OHP in the urine of the exposure group [0.725, 0.300, 0.092 and 0.059 μg / mmo L, respectively, Were significantly higher than the control group [0.615,0.197,0.065,0.040μg / mmo L (Cr)] (P <0.01); stepwise logistic regression analysis showed that every increase of smoking level caused 2-NAP high The possibility of concentration increased by 3.393 times. The possibility of high concentrations of 2-NAP, 2-FLU, 9-PHE and 1-OHP in the contact group were 1.665,3.399,2.185 and 2.905 times that of the control group, respectively. [Conclusion] The main factors influencing the concentration of metabolites of PAHs in the urine are type of work and smoking. Among them, 2-FLU and 1-OHP are biomarkers sensitive to occupational exposure of coke oven workers, while 2-NAP is a sensitive organism exposed to smoking Sign.