论文部分内容阅读
目的掌握江西省外环境中霍乱弧菌菌型分布、毒力基因、耐药现状,为霍乱防治提供科学依据。方法采用血清学分型法对外环境中分离的霍乱弧菌进行分型,同时以主要毒力基因ctx为引物对检索的霍乱弧菌进行PCR扩增,并应用K-B纸片法对部分菌株进行10种抗生素的药物敏感试验。结果 2009-2012年期间,共从外环境中分离到霍乱菌株156株。毒力实验结果显示37株O139群中15株扩增出与阳性对照一致的毒力基因(ctx)条带,而119株O1群除2株小川型外,其余均未扩增出毒力基因(ctx)条带,与阴性对照一致。药敏结果显示:O139群和O1群对丁胺卡那霉素、诺氟沙星、环丙沙星、头孢噻肟90%以上敏感。结论江西省2009-2012年间外环境中分离到的霍乱弧菌中,O139群与O1群并存,O1群为优势菌;水产品中O139群菌株绝大多数为产毒株,而O1群菌株绝大多数为非产毒株。应重点加强水产品的霍乱监测。药敏结果供霍乱治疗和必要时的预防药物选择提供依据。
Objective To understand the distribution of Vibrio cholerae, virulence genes and drug resistance in the external environment of Jiangxi Province and provide a scientific basis for the prevention and treatment of cholera. Methods Vibrio cholerae isolated from the external environment were genotyped by serological typing method. Vibrio cholerae were amplified by PCR using ctx as the major virulence gene and 10 strains Antibiotic drug sensitivity test. Results A total of 156 cholera isolates were isolated from the external environment during 2009-2012. Toxicity test results showed that 15 out of 37 O139 groups amplified virulence gene (ctx) bands consistent with the positive control, while 119 O1 groups except for 2 Ogawa type, the other did not amplify virulence genes (ctx) band, consistent with the negative control. Susceptibility results show: O139 group and O1 group of amikacin, norfloxacin, ciprofloxacin, cefotaxime more than 90% sensitive. Conclusions Among Vibrio cholerae isolates isolated from the external environment in Jiangxi province from 2009 to 2012, O139 group and O1 group coexist and O1 group is the predominant bacteria. Most of the O139 group strains in aquatic products are toxigenic strains, while the O1 group isolates Most non-toxic strains. Cholera surveillance of aquatic products should be emphasized. Susceptibility results provide a basis for cholera treatment and preventive drug selection where necessary.