本文探讨了灵芝 (Ganodermalucidum)菌丝原生质体的制备及再生的条件。实验结果表明 :采用MYG为灵芝菌丝培养基 ,2 %真菌溶壁酶 (Lywallzyme)作脱壁酶 ,0 6mol/L甘露醇作渗透压稳定剂 ;对培养 6d的灵芝菌丝在 30℃下进行酶解 ,酶解时间 4- 6h ,可产生约 3 0× 1 0 6个 /mL (0 1g菌丝 )原生质体 ,并在添加灵芝子实体浸出汁的双层培养基上实现了灵芝菌丝原生质体的再生。 This article explored the preparation of Ganoderma lucidum mycelium protoplasts and the regeneration conditions. The experimental results showed that: Mycoplasma ganoderma lucidum mycelium culture medium, 2% fungi lywallzyme (Lywallzyme) as wall-releasing enzyme, 0 6mol / L mannitol as osmotic pressure stabilizer; cultured 6d Ganoderma lucidum mycelium at 30 ℃ After enzymolysis and enzymolysis for 4-6h, protoplasts of about 30 × 106cells per mL (0 1g mycelium) were produced, and Ganoderma lucidum Regeneration of silk protoplasts.