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在聚酰胺膜 ( PM)等固体基质上 ,人免疫球蛋白 ( Ig G)能与异硫氰酸荧光素 ( FITC)标记的羊抗人抗体( GAHAb* )发生定量的特异性免疫反应 ,并保持了 FITC优良的室温磷光特性 ,据此建立了固体基质室温磷光免疫分析 ( SS-RTP-IA)测定人血清中人 Ig G的新方法 .在 0 .4μL(取样浓度为 1 .5 6～ 5 0 ng/m L)点样体积内 ,人 Ig G的含量在 0 .62 4～ 2 0 pg范围内 ,与室温磷光强度呈良好的线性关系 ,工作曲线的回归方程 ΔIp=31 .4 +4 6.2 lg[c( Ag) /pg],n=6.相关系数 r=0 .999,检出限 0 .32 pg.用于正常人血清中人 Ig G含量的测定 ,加标回收率为 96%～ 1 0 4 % ,结果满意 .
Human immunoglobulins (Ig G) can be quantitatively and specifically immunoreactive with fluorescein isothiocyanate (FITC) -labeled goat anti-human antibody (GAHAb *) on a solid substrate such as a polyamide membrane The new method of determination of human Ig G in human serum by solid-phase room-temperature phosphorescence immunoassay (SS-RTP-IA) was established by keeping the good room-temperature phosphorescence property of FITC.Under the condition of 0.4 μL (sample concentration 1.56 ~ 5 0 ng / m L), the content of human Ig G was in the range of 0.62 4 ~ 2 0 pg, and showed a good linear relationship with the room temperature phosphorescence intensity. The regression equation of the working curve ΔIp = 31.4 + 4 6.2 lg [c (Ag) / pg], n = 6. The correlation coefficient r = 0.999, the detection limit of 0.32 pg for the determination of human serum Ig G levels, the standard recovery was 96% ~ 104%, the result is satisfactory.