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为了探讨羌塘自然保护区高寒草地土壤微生物数量组成及获得新药物先导化合物,选择土壤质地、植被类型基本一致,海拔高度不同的8个样地,采用稀释平皿分离测数及琼脂块法研究了土壤微生物数量及与生态因子的相关性、微生物酶活性和拮抗性。结果表明:研究区域微生物总数量为细菌占绝对优势,其次是放线菌,真菌数量最少。相关性分析表明,海拔高度、有机质与微生物各类群均具有显著或极显著负相关性,土壤含水量与微生物各类群呈正相关,除真菌与pH值呈负相关外,其他微生物类群与pH值呈正相关,且达到了极显著水平。羌塘自然保护区高寒草地土壤微生物产酶活性较强,但拮抗性较弱,揭示了从碱性高寒草甸土中筛选到高拮抗活性菌株的可能性较小。
In order to investigate the composition of soil microorganisms and to obtain new lead compounds in alpine grassland of Qiangtang Nature Reserve, 8 samples with different soil texture, vegetation types and different altitudes were selected and analyzed by dilution plate assay and agar block method Soil microbial quantity and its relationship with ecological factors, microbial enzyme activity and antagonism. The results showed that the total number of microorganisms in the study area was the dominant bacteria, followed by actinomycetes, with the least amount of fungi. Correlation analysis showed that there was a significant or extremely significant negative correlation between soil organic matter and microorganism in all altitudes, and the soil water content was positively correlated with the microbial populations. In addition to the negative correlation between fungi and pH value, Value was positively correlated, and reached a very significant level. The alpine grassland in Qiangtang Nature Reserve had stronger enzyme activity, but weaker antagonism, which indicated that it was less likely to screen highly active strains from alkaline alpine meadow soil.