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目的 :研究内皮型一氧化氮合酶 (eNOS)与兔实验性血管痉挛的关系。方法 :2 8只新西兰白兔分成假手术对照组 (8只 )、手术加普通饮食组 (8只 )和手术加高胆固醇饮食组 (12只 )。血管造影观察球囊内皮剥脱前后和分别给予普通饮食和高胆固醇饮食喂养 8周后麦角新碱诱发血管痉挛情况。硝酸还原酶法测定各组血浆一氧化氮 (NO)水平 ,免疫组化检测各组血管eNOS含量。结果 :血管造影显示手术加高胆固醇饮食组可诱导出局限性血管痉挛。免疫组化显示eNOS定位在血管内皮细胞胞质、痉挛血管节段内皮细胞内表达减少。手术加高胆固醇饮食组血浆NO的含量也明显下降。结论 :高胆固醇和内皮剥脱使eNOS合成下调 ,血管eNOS含量降低可能与实验性血管痉挛的诱发高度相关
Objective: To investigate the relationship between endothelial nitric oxide synthase (eNOS) and experimental vasospasm in rabbits. Methods: Twenty-eight New Zealand white rabbits were divided into sham-operation control group (n = 8), operation plus normal diet group (n = 8) and surgery plus high cholesterol diet group (n = 12). Angiography was performed to observe ergometrine-induced vasospasm before and after exfoliation of the balloon and after 8 weeks of normal diet and high-cholesterol diet respectively. Nitric acid reductase method was used to measure the level of plasma nitric oxide (NO) in each group. The content of eNOS in each group was detected by immunohistochemistry. Results: Angiography showed that surgical hypercholesterolemia induced localized vasospasm. Immunohistochemistry showed that eNOS was localized in the cytoplasm of vascular endothelial cells and the expression of eNOS was decreased in the vascularized vascular endothelial cells. The level of plasma NO in the surgery-plus-cholesterol diet group also dropped significantly. CONCLUSIONS: High cholesterol and endothelial ablation down-regulated eNOS synthesis, and decreased eNOS content in blood vessels may be highly correlated with the induction of experimental vasospasm