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利用国家蔬菜种质资源库的1904份辣椒资源材料,采用测序技术获得eIF4E(eukaryotic translation initiation 4E)基因exon1序列,研究e IF4E基因多样性及我国辣椒种质资源群体多样性。结果表明:在1904份材料中共发现17个单倍型,14个有义多态性位点,其中9个为新的位点,位点大多集中在eIF4E蛋白表面环上;8个地理群体的平均单倍型多样性(Hd)和平均核苷酸多样性(Pi)分别为0.519和0.00210;群体间分化指数(Fst值)及基因流(Nm)表明不同群体间表现差异的分化程度;AMOVA分析表明总变异主要来源于群体内个体间的变异(97.23%),只有2.77%变异发生在群体间。本研究将有助于了解我国辣椒eIF4E基因多样性,为抗PVY育种提供更多抗源材料。
Using 1904 capsicum resource materials from National Vegetable Germplasm Bank, the exon1 sequence of eIF4E (eukaryotic translation initiation 4E) gene was obtained by sequencing, and the diversity of e IF4E gene and the population diversity of pepper germplasm in China were studied. The results showed that: 17 haplotypes and 14 sense polymorphic sites were found among 1904 materials, of which 9 were novel sites, most of which were located on the surface of eIF4E protein surface; The mean haplotype diversity (Hd) and average nucleotide diversity (Pi) were 0.519 and 0.00210, respectively. Differentiation index (Fst) and gene flow (Nm) between groups indicated the differentiation degree of different groups. AMOVA The analysis showed that the total variation mainly came from the intra-group variation (97.23%) and only 2.77% variation occurred among the populations. This study will help to understand the diversity of pepper gene eIF4E in China and provide more anti-source materials for breeding PVY.