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目的：在临床研究过程中,我院介入放射科采用将丝裂霉素加入到碘化油内加热后,注入局部肿瘤栓塞的方法,治疗中、晚期肝癌患者。因此,丝裂霉素加热后的含量变化是迫切需要解决的问题。本实验应用一个高效、灵敏的HPL仪器,紫外检测器法,测定了丝裂霉素在28℃、40℃、50℃、60℃、70℃、80℃、90℃、100℃的含量变化情况,为临床合理用药提供理论依据。方法:采用Waters Symmetty C184.6mm(ID)×250mm(L)色谱柱,31％甲醇、69％醋酸钠缓冲液为流动相,检测波长为365nm,流速为1ml/min。仪器:高效液相,紫外检测器。结果:丝裂霉素在28℃、40℃、50℃、60℃、70℃、80℃、90℃、100℃的含量下降百分率分别为0.02％、0.59％、0.58％、7.88％、9.08％、9.47％、9.97％、10.15％;重复进样,其相对标准差(RSD)小于2.0％,标准曲线R=0.9949。结论:丝裂霉素溶液随着温度的升高,含量逐渐下降。
OBJECTIVE: In the course of clinical research, the interventional radiology department of our hospital adopted the method of adding mitomycin into heating iodine oil and injecting local tumor embolization method to treat patients with advanced liver cancer. Therefore, mitochondria content changes after heating is an urgent need to solve the problem. In this experiment, an efficient and sensitive HPL instrument, UV detector method was used to determine the content of mitomycin at 28 ℃, 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃, 90 ℃, 100 ℃ , To provide a theoretical basis for clinical rational use of drugs. Methods: The mobile phase was Waters Symmetty C18 4.6mm (ID) × 250mm (L) column, 31% methanol and 69% sodium acetate buffer. The detection wavelength was 365nm and the flow rate was 1ml / min. Instrument: HPLC, UV detector. Results: The mitochondrial percentage of mitomycin decreased by 0.02%, 0.59%, 0.58%, 7.88% and 9.08% at 28 ℃, 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃, 90 ℃ and 100 ℃ respectively. , 9.47%, 9.97% and 10.15% respectively. The relative standard deviation (RSD) was less than 2.0% with repeated injections. The standard curve R = 0.9949. Conclusion: Mitomycin solution with the temperature increased, the content decreased.