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采用根系分隔试验,研究不同施氮水平(0.1、0.3、0.5和0.7g·kg-1)下,玉米-大豆间作系统中根系互作对玉米植株生长、产量和根际微生物的影响.结果表明:根系互作和增施氮肥可以增加玉米株高、叶片长和叶片宽,提高玉米叶绿素含量.未隔根处理玉米成熟期的根干质量与隔根处理相比差异不显著.在0.1、0.3、0.5和0.7g·kg-1施氮水平下,未隔根处理的单株生物量分别增加8.8%、6.3%、3.6%和0.7%,单株经济产量分别增加17.7%、10.0%、8.2%和0.9%.未隔根处理真菌和固氮菌数量与隔根相比显著增高;随着施氮水平的提高,根际细菌、真菌和放线菌的数量均呈逐渐增加趋势,而固氮菌的数量呈先增加后下降的趋势.玉米成熟期的根冠比与细菌、真菌和放线菌数量呈显著负相关,与固氮菌数量相关性不显著.根系互作有利于改善玉米植株生长,增加玉米产量和根际微生物数量,但其效果会随供氮水平的提高而减弱.
The effects of root interaction on the growth, yield and rhizosphere microbes of maize plants under different N rates (0.1, 0.3, 0.5 and 0.7g · kg-1) were studied by root segregation test.The results showed that: Root system interaction and nitrogen application increased maize plant height, leaf length and leaf width, and increased chlorophyll content of maize.The root dry weight of corn without maturity was not significantly different from that of isolation treatment at 0.1, 0.3, Under the nitrogen application rates of 0.5 and 0.7g · kg-1, the biomass of single plant without root isolation increased by 8.8%, 6.3%, 3.6% and 0.7%, and the economic yield per plant increased by 17.7%, 10.0% and 8.2% And 0.9%, respectively.The number of fungi and nitrogen-fixing bacteria in non-root-isolated groups was significantly higher than that in isolated roots.The numbers of rhizobacteria, fungi and actinomycetes tended to increase with the increase of nitrogen application level, The number increased first and then decreased.The root / shoot ratio of maize was negatively correlated with the number of bacteria, fungi and actinomycetes, but not with the number of nitrogen-fixing bacteria.The interaction of root system was beneficial to improve the growth of maize plants, Corn yield and rhizosphere microbial quantity, but the effect will be with the supply Increased levels weakened.