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目的:以高效液相色谱法(HPLC)测定大田基黄中槲皮素含量,并优化大田基黄总黄酮的提取工艺。方法:高效液相色谱法测定大田基黄中槲皮素含量,色谱柱为ZORBAXSB-C18(250mm×4.6mm,5μm),流动相:甲醇-0.20%冰醋酸水溶液(42:58),流量1.0mL/min,检测波长360nm,柱温25℃,进样量20μL;以总黄酮含量为考察指标,采用正交设计的方法研究提取大田基黄总黄酮的工艺条件。结果:HPLC法分析槲皮素时色谱峰的峰面积与含量呈良好的线性关系(r=0.9964),大田基黄中槲皮素含量3.25mg/g。乙醇浓度60%,温度60℃,料液比1:30,提取时间1h,提取2次时大田基黄总黄酮得率可达3.83%。结论:该方法对大田基黄黄酮类化合物的研究开发具有一定的指导意义。
Objective: To determine the content of quercetin in Datianjihuang by high performance liquid chromatography (HPLC), and to optimize the extraction process of total flavonoids in datianjihuang. Methods: The content of quercetin in Datianjihuang was determined by high performance liquid chromatography. The chromatographic column was ZORBAXSB-C18(250mm×4.6mm, 5μm). The mobile phase was methanol-0.20% aqueous acetic acid solution (42:58). The flow rate was 1.0mL/. Min, the detection wavelength was 360nm, the column temperature was 25°C, and the injection volume was 20μL. With the content of total flavonoids as the inspection index, the orthogonal design method was used to study the extraction conditions of total flavonoids from Datianjihuang. Results: The chromatographic peak area and content of quercetin showed a good linear relationship (r=0.9964). The content of quercetin in Dajijihuang was 3.25 mg/g. Ethanol concentration 60%, temperature 60 °C, solid-liquid ratio 1:30, extraction time 1h, extraction twice, Datianjihuang total flavonoids yield up to 3.83%. Conclusion: This method has certain guiding significance for the research and development of Datian yellowflavonoids.