目的　构建含蛋白转导结构域 (PTD)与慢性粒细胞白血病 (CML)bcr/abl融合基因片段的质粒 ,并在大肠杆菌中表达。方法　PCR扩增的CMLbcr/abl基因片段 ,经DNA测序后 ,与合成编码PTD的DNA片段一起插入质粒pET 16b ,构建表达载体pEPb ,转化大肠杆菌并进行了PTD bcr/abl蛋白的诱导表达和纯化。结果　跨越bcr/abl断裂点的 5 2 3bp的目的片段被有效地扩增。DNA序列分析表明所构建的含PTD bcr/abl融合基因的质粒与设计相同。PTD bcr/abl融合蛋白在转化大肠杆菌获得了高效表达并纯化。结论　成功地获得了PTD bcr/abl融合蛋白片段的基因表达产物 ,为进一步研究CML的免疫治疗奠定了基础 Objective To construct a plasmid containing the bcr / abl fusion gene of protein transduction domain (PTD) and chronic myeloid leukemia (CML) and express in Escherichia coli. Methods The CMLbcr / abl gene fragment amplified by PCR was inserted into plasmid pET 16b with the DNA fragment encoding PTD after DNA sequencing. The expression vector pEPb was constructed and transformed into Escherichia coli. The induced expression and purification of PTD bcr / abl protein . Results The 5 2 3bp target fragment spanning the bcr / abl breakpoint was efficiently amplified. DNA sequence analysis showed that the constructed plasmid containing PTD bcr / abl fusion gene was identical to the designed one. The PTD bcr / abl fusion protein was highly expressed and purified in E. coli. Conclusion The gene expression products of PTD bcr / abl fusion protein fragment were successfully obtained, which laid the foundation for further study of immunotherapy of CML